الفهرس 
Relationship between mastitis and mycoplasma bovis
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Abstract
Mycoplasma species were the simplest form of self-replicating organism. Mycoplasma is one of the causative agents causing mastitis in cattle, buffaloes which alone or together with viruses and/or bacteria cause important specific disorders. The present study aims to Isolation and serological identification of mycoplasma strains from milk samples. During this study 350 milk samples collected from some private farms from Elfayoum governate of dairy cows and buffaloes (50ml each) collected aseptically in sterile McCartney bottles. The results of Mycoplasma species isolation in cattle from clinical mastitic samples (58%), followed by subclinical mastitis samples (16%) and finally bulk milk tank samples (12%). in buffaloes, clinical mastitic samples (36%), subclinical mastitis samples (10%), and finally bulk milk tank samples (8%). In this study we used digitonin sensitivity test to differantiate between mycoplasma and acholeplasma genera, In cattle from clinical mastitic 89.7% were mycoplasma and 10.3% were acholeplasma. In subclinical mastitis samples 93.8% were mycoplasma and 6.2% were acholeplasma, in bulk milk tank samples 100% were mycoplasma and 0% were acholeplasma In the buffaloes from clinical mastitic samples 94.4% were mycoplasma and 5.6% were acholeplasma. In subclinical mastitis samples 90% were mycoplasma and 10% were acholeplasma, finally in bulk milk tank samples 100% were Mycoplasma and 0% were acholeplasma. The morphological, biochemical and genetically tests were applied on pure isolates. The pure isolates (Mycoplasma spp.) were applied with MIC technique such as (spiramycin and tilmicosin) and immunosorbent assay (ELISA) was used for the detection of M. bovis antibodies in milk samples of cattle and buffaloes