الفهرس 
Assessment of the gossypol content extracted from different Egyptian cotton seed varieties and studying its effect on metabolic and key organ function in mice
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Abstract
Gossypol, C30 H30 O8, is a highly reactive polyphenolic compound derived from the cotton plant. Gossypol content of cotton plants has become a big issue both scientifically and commercially due to its unique biological characteristics and influence of its toxicity on use of animal feed and human consumption. The present study includesd 52 mice which were divided into four groups: control group and three treated groups, that were injected through interperitonial injection for one month with gossypol acetic acid GAA by a doses of (2.5, 5, 7.5 mg/kg BW, respectively). Also in the present study the gossypol content of Egyptian cotton seed from the varieties Giza 86, Giza88, Giza 90 and Giza 92 were assessed through an optimized HPLC on a C18 column (406 mm x 250 mm, 5æm particle) with methanol-0.5 acetic acid aqueous solution, 90: 10 (v/v), as mobile phase, at a flow rate of 0.8 ml/min and UV detection at 254 nm. The objective of this study was to determine the effect of gossypol on different blood biochemical profiles, as well as the investigation of the oxidative properties caused by gossypol. Also this work aimed to assess the gossypol content present in different Egyptian cotton seed varieties using high performance liquid chromatography technique (HPLC). Treatment with GAA was found to cause a significant increase in the activity of serum transaminases, alkaline phosphatas, lactate dehydrogenase, total protein, hepatic glutathione, uric acid, creatin kinase and sodium in all treated groups with highly significance in the dose of 7.5 mg kg BW. While the value of albumin, malondialdehyde, cholesterol,potassium and calcium were significantly decreased in all treated groups with highly significant decrease in the dose of 7.5 mg kg BW. Creatinine , urea and phosphorous showed insignificant difference in all treated groups