الفهرس 
Prospects for human stem cell therapy in the treatment of end stage liver cirrhosis in rats
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Abstract
responding author: 01112231511, Egypt, 11611, eman.50.fawzy@gmail.com
Abstract
Aims: to assess immunohistochemically the expression of CD38 in induced rat liver
fibrosis after treatment with mesenchymal stem cells (MSCs) and validate their
therapeutic potential.
Study design: induction of fibrosis in liver of rats by using carbon tetrachloride
(CCl4) then treatment with bone marraw MSCs (BM-MSCs). .
Place and Duration of Study: National cancer institute, Cairo University.
Methodology: Forty five adult rats were divided into 3 groups: control group A,
CCl4-indused fibrosis group B and CCl4/BM-MSCs group C. After one month from
transplantation, histological and immunohistochemistry examination of the liver
tissue were performed.
Results: Immunohistochemical staining for CD38 in liver showed negative cell
membrane reactivity (0) in peri-portal and peri-venular areas in control group A. The
CCl4-untreated rats (group B) without stem cell treatment showed moderate cell
membrane reactivity (++) of hepatocytes peri-venular area. The CCl4-untreated rats
with stem cell treatment (group c) showed negative cell membrane reactivity (0) for
CD38 in peri-portal and peri-venular areas, CD38 expression semi-quantitatively
analyzed by an experienced liver pathologist using a scoring system ranging from 0 to
+3, where 0 indicates no expression of CD38 and +3 indicates the highest expression.
Conclusion: The treated liver showed a degree of improvement of CCL4 induced
hepatic injury, with decreased expression for CD38 in liver tissue when compared to
the fibrotic livers.
Keywords: CD 38, fibrosis, mesenchymal stem cell, animal model, CCl4
Abbreviations: mescenchymal stem cells (MSCs), cluster of differentiation (CD38),
carbon tetrachloride ( CCL4)