الفهرس 
cover englishOnly 14 pages are availabe for public view
 |  | from | 126 |  |  |
| | | from | 126 | | |
Abstract
A total of 101 pet dog samples (67nasal swabs and 34 wound swabs) were collected from 101 pet dogs. All samples were cultured for the isolation of Staphylococcus aureus using selective media and biochemical tests. Isolates were identified as MRSA after antimicrobial susceptibility testing. PCR for mecA gene was applied. Nineteen MRSA isolates were recovered from 67 (28%) nasal swabs and 6 wound isolates from 34 (17.6%). All isolates were resistant to cefoxitin and sensitive to both polymyxin B and vancomycin. All the 25 MRSA isolates were positive to mecA gene. A total of 53 pet cats 42 oropharyngeal swabs and 11 rectal swabs were collected. A total of 24 nasal swabs from human. All samples were cultured for the isolation of Staphylococcus aureus using selective media and biochemical tests. Isolates were identified as MRSA after antimicrobial susceptibility testing. PCR for mecA gene was applied. The investigation of MRSA in cats oropharyngeal and rectal swabs revealed (58.8 & 50%) respectively, for human was 21.4%. All isolates were resistant to cefoxitin and sensitive to both polymyxin B and vancomycin. Thirteen PCR amplicons from both cats & human were subsequently recognized by molecular analysis of spa gene polymorphism in clinical isolates by PCR-RFLP. The data were analyzed by GENEANALYZER software for actual analyzing and determining of amplicons size after HAEII restriction enzyme digestion of spa PCR product. A total of 8 different electrophoretic patterns were observed for the HaeII digested PCR products among the 13 strains of MRSA.