الفهرس | Only 14 pages are availabe for public view |
Abstract The main aim of this study is to apply phenotypic and genotypic characterization of members of Pasteurella spp. isolated from camels from different governorates in Egypt. Camels with respiratory manifestation as nasal discharge, fever, off food, with ocular discharge and air gasping, paralysis of lips and salivation were subjected to clinical examination and a total of 141 samples were collected as follow; 111 nasopharyngeal swabs, 15 tracheal swabs and 15 lung specimens collected from camels from different governorates including New valley, Giza, Naj Hammadi (Qena governorate), Halaib and Shalateen (Halaib Triangle), the samples were subjected to isolation and identification of Pasteurella spp. by conventional phenotypic method and genotypic by polymerase chain reaction (PCR). At the level of biochemical characterization, the incidence of Pasteurella multocida was 40.4%, and Mannheimia haemolytica was 7.1%. Using kmt1 gene for identification of the isolates for P. multocida and sse gene for identification of the isolates for M. haemolytica, the results revealed that six isolates showing positive PCR for P. multocida, M. haemolytica could not be detected by PCR. Sequencing of kmt1 gene of the six isolates showing a great homology with other P. multocida strains uploaded in genebank, the phylogenetic tree revealed clear clustering of the six isolates with other P. multocida ancestors uploaded in genebank. Identification and characterization of Pasteurella spp. isolated from camels improve diagnosis, epidemiology and treatment of the organism causing diseases. |