الفهرس 
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Abstract
Summary
The diseased Chickens were collected and the history recorded from different commercial Chickens farms of Qaluybia , El Sharkia , Gharbia , and El Monofia governorates depending distinct NDV like viral symptoms from January to May 2019 and 2020. Newcastle disease (ND) was detected depending clinical signs of NDV like viral symptoms and serological test by chromatographic Immunoassay .Virus was isolated from kidney , liver, proventriculus and spleen and inoculated in embryonated chicken eggs . The allantoic fluid was harvested from inoculated chicken eggs . NDV isolates detected by Heamagglutination and Haemagglutination inhibition tests . The biological properties of NDV isolates were determined . Biochemical of blood of infected with the velogenic NDV isolate was analyzed and the longevity , UV light , pH and some disinfectant chemicals on the survival of NDV isolates were determined .
Application of probiotics feed additives on NDV infected chickens were recorded on body weights , analysis of biochemical blood , ,total serum protein , serum albumin , total proteins and calculation of serum globulin fractions.
The obtained results : .
NDV isolates were detected in commercial chickens based clinical signs and related to the rick factors, age, mortality %, programmer of vaccination and confirmed by chromatographic Immunoassay test.
Nineteen diseased chickens out of 95 collected diseased chickens were recorded . Prevalence of chicken virus disease in Qalubyia were (5 / 25) , El Sharkia (5 / 15) , El Monofia (7 / 35) , and El Gharbia (5 / 25) governorates .
The nine diseased chicken isolates (namely ND1, ND2, ND3, ND5, ND6, ND7 , ND9 , ND10 & ND13 isolates) had heamagglutinating activity were cultivated on allantoic fluids in the chicken embryo eggs at 9-11 days . The nine isolates were divided into 3 categories , severe isolates ND3 , ND5 & ND6 were caused death of 5 from 5 chicken embryo after 72, 54, 84 hrs. respectively . Moderate severe isolates , ND1 , ND7 , ND9 & ND10 were caused death of 4 from 5 chicken embryo after 84, 94, 85 , 95 hrs. respectively . Mild severe isolates , ND3 & ND13 were caused death of 3 from 5 chicken embryo after 104 and 148 hrs. respectively .
The died embryo were examined for the internal signs to confirm that the death due to the virus of Newcastle disease . As shown in the sever haemrrhages were observed around the embryo and on brain region . The virus isolates No. (ND 4, ND 8 , ND 11, ND 12, ND 14 & ND 15) inoculated in the chicken embryo gave negative results in HA test .
The nine isolates (ND1, ND2 , ND3 , ND5 , ND6 & ND7 ND9 , ND9, ND10 & ND13) gave different titers of NDV virus .While six isolates ( (ND 4, ND 8 , ND 11, ND 12, ND 14 & ND 15) gave negative results with HIA and HA tests .