الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 132 |  |  |
| | | from | 132 | | |
Abstract
Staphylococcus aureus Bacteria is the main cause to human pathogen . Its present in human and mammals. Its gram positive cocci that have resistance to different antibiotics. the incidence of multidrug resistant bacteria represent serious threat to public health. Some bacteria that are capable of causing serious disease are becoming resistant to most commonly available antibiotics. Antibiotic resistant bacteria can spread from person to person in the community or from patient to patient in hospital. So that the present study aimed to find out to detect pathogenicity and biofilm formation of s.aureus and find out alternative methods for the treatment other than the traditional antibiotic treatment.
The obtained results can be summarized as following:
1) In this study 79 samples were collected from patients who have infections at the Clinics of Benha University Hospital, Egypt. The seventy nine samples have been isolated from forty male patients and thirty nine female patients with ages ranged from 1 month to over than sixty years.
2) Swabs were streaked on blood agar and mannitol salt agar media under aerobic conditions at 37°C for isolation.
3) Preliminary definition of all bacterial isolates isolated from patients infections using Gram stain and using traditional biochemical tests.
4) The results showed that the percentage of distribution are found in Staphylococcus aureus was found (72.1%).
5) Identification of S.aureus isolates by 16S rRNA.
6) Staphylococcus aureus isolates were tested against different antibiotic discs. was susceptible to Vancomycin (VA, 30mg), and 63.16% were sensitive to Ciprofloxacin (CRO, 30mg). Then, oxacillin (OX, 5µg) was active against 42.11 % of the isolates.
7) Staphylococcus aureus isolates (100%) were resistant to Cefotaxime (CTX,30mg), Amoxicillin-clavulanic acid (AMC, 30mg), cefoxitin(FOX, 30mg) and Ceftriaxone (CRO, 30mg). Additionally, 84.21% of the isolates were resistant to penicillin-G (P, 10 µg ), and Trimethoprim sulfamethoxazole(Sxt, 25mg), whereas 87.72% of the isolates were resistant to Gentamycin(GM, 30mg).
8) Detection of pathogenicity and biofilm formation of S.aureus isolate which are virulence (Agr), biofilm formation (icaA), β-lactams resistance (blaTEM), aminoglycosides resistance (aadA1), and methicillin resistance (mecA), were investigation for presence and absence in selected 12 strains of S. aureus
9) Application of ten Eos (sage oil, nigella sativa oil, oregano oil, lemon oil, linseed oil, thyme oil, moringa oil, tea oil, garlic and ginger oil) on two s.aureus isolates detect strong antimicrobial activity of Eos on S.aureus.