الفهرس 
Assessment of salivary nitric oxide levels and dental caries experience in children with congenital heart disease :Cross sectional study
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Abstract
Background: Nitric oxide (NO) is a highly reactive radical, participates in nonspecific natural defense mechanism of the oral cavity. The aim of this study was to assess salivary NO level and dental caries experience in children with CHD in comparison to healthy children and to establish a correlation between salivary nitric oxide level and dental caries. Subjects and Methods: This cross-sectional study carried on 155 cardiac patients of Cairo University Paediatric Hospital and 155 healthy patients attending the Paediatric Dentistry Department, Faculty of Dentistry, Cairo University. Dental caries of each child was assessed using WHO criteria for diagnosis of dental caries and dental caries indices for primary (dmft), mixed (deft) and permanent dentition (DMFT). Salivary samples were collected and kept in well-sealed plastic tubes then refrigerated. Salivary NO was measured as total nitrate (NO3) and nitrite (NO2) using Griess reagent and vanadium trichloride (VCL3) then the samples were analysed using spectrophotometric microplates assay reader. Results: CHD group showed statistically significant higher mean salivary NO level than control group. CHD group showed higher mean dmft, DMFT and deft scores than control group. Cyanotic CHD group showed higher mean scores of dmft in deciduous dentition than acyanotic CHD group. Cyanotic CHD group were similar in caries index mean scores to acyanotic in mixed and permanent dentition stages. Cyanotic CHD group showed higher mean level of NO than acyanotic. There was a statistically significant direct correlation between DMFT and salivary NO level. However, there was no statistical significant correlation between dmft, deft and NO levels