الفهرس 
Laser based detection of maternal plasma cell free fetal DNA for early fetal sex determination
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Abstract
Enhancement of DNA detection assays increases the use of non-invasive prenatal diagnosis that consequently enhances the supervision plan of pregnant woman with genetic disorder that could have an expected babies with this disorder. Aim of work: current work was planned to examine plasma of pregnant women starting from 6 th week of the pregnancy for Cell-free fetal DNA, to determine embryonic sex type with consequent prospect of detection of fetal anomalies such as sex-linked genetic abnormalities . Method: samples collected from 6 th week till 11 th week of pregnancy according to pregnancy age and tested using Real time PCR for determination of fetal sex Results: testing of samples resulted in detection of fetal sex starting from 6th week and the later the gestational age ,the better the result for detection of fetal sex . all results were confirmed by Ultrasound scan and neonatal outcome. Testing results revealed PCR detection for 58 males and 92 females with confusion in one fetus due to non identical twins