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العنوان
Biochemical studies of some plants as antioxidant and anticancer /
الناشر
Dalia Ahmed Hashim Mohamed ,
المؤلف
Dalia Ahmed Hashim Mohamed
هيئة الاعداد
باحث / Dalia Ahmed Hashim Mohamed
مشرف / Fouad Abdelrehim Ahmed
مشرف / Amr Ahmed Mostafa
مشرف / Nermien Zakaria Ahmed
تاريخ النشر
2018
عدد الصفحات
146 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
الزراعية والعلوم البيولوجية (المتنوعة)
تاريخ الإجازة
25/9/2018
مكان الإجازة
جامعة القاهرة - كلية الزراعة - Agricultural Biochemistry
الفهرس
Only 14 pages are availabe for public view

from 170

from 170

Abstract

Part one:This present study was designed to investigate the antioxidant activity of three ethanolic extracts of Annona squamosa (leaves),Coffea arabica (beans) and Moringa oleifera (leaves) In-vitro. 1,1-diphenyl-2-picryl-hydrazyl (DPPH), Hydroxyl radical scavenging (OH{u2022}), Ferric reducing antioxidant power (FRAP), Total antioxidant capacity (TAC),Catalase (CAT), and Peroxidise enzymes (POX). Total phenolic (TP), Total flavonoids (TF) and Total soluble protein (TSP) contents were determined. Also, the effects on lysosomal enzymatic activities of the three extracts and Rutin hydrate (standard) by two doses of each extract as compared to control group on the four marker lysosomal enzymatic activities namely: acid phosphatase ”ACP”; Ý-galactosidase”Ý-GAL”; Ý-N-acetyl glucoseaminidase”Ý-NAG”, and Ý-glucuronidase ” Ý-GLU” after 60 and 120 minutes of incubation in rat liver were investigated.The chemical profile and the contents of each extract by HPLC method were also determined. Part two:The potential anti-proliferation properties of the three ethanolic extracts on human breast carcinoma cell line type (MCF-7) was investigated using neutral red uptake assay for the estimation of the cell viability.The results showed that, the most potent antioxidant activity was observed for Coffea arabica (beans) then Moringa oleifera (leaves) followed by Annona squamosa (leaves) and the most potent inhibitory effect on lysosomal enzymatic activities was observed for Moringa oleifera leaves) then Coffea arabica (beans) followed by Annona squamosa(leaves) as compared to Rutin hydrate which were dose and enzyme type dependent