الفهرس 
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Abstract
There is a growing necessity for herbal medicine usage in treatment because herbs have fewer side effects compared with synthetic drugs. In addition, the emergence of resistance by different pathogenic bacteria and parasites to the currently used chemicals has been reported. There are increasing needs for more effective and safer parasiticides, anti-inflammatory and anti-microbial agents. Jasminum grandiflorum L. is a medicinal plant widely used in the traditional medicine as an anthelmintic in ringworm infections, for treating ulcers, stomatitis, skin diseases, and wounds. Our study has renewed the interest in the genus Jasmine for the treatment of parasitic infestation and chronic inflammatory conditions as well. The total methanol extract of Jasminum grandiflorum L. aerial parts was tested for its therapeutic potential as anthelmintic, anti-inflammatory, anti-microbial and immune-stimulant effects to confirm its traditional uses. Whole plant of Jasminum grandifloroum methanol extract (JGTE) was prepared and partitioned into dichloromethane (DCM-F) and n-butanol (BuOH-F) fractions. Kaempherol neohesperoside (1), rutin (2), oleuropein (3), and ligstroside(4) and were isolated, identified and tested in vivo for their anthelmintic effect. Moreover, the total methanol extract of Jasminum grandifloroum was tested in vitro for anthelmintic activity using two nematodes; one larval stage of cestode and one arthropod. The tested parasites were Habronema muscae and Parascaris eqourum adults and Hydatid cyst from 130 donkeys slaughtered at zoo abatoir, and Pediculushumanus capitis from school children. Parascaris eqourum, were exposed to upgrade concentration of 50, 100, 200, 400, 600, 800, 1000 and 1200 ppm., each for 6, 12, 24 hours exposure time in saline solution. Concentrations which induce 50% mortalities or above were re-screened at lower exposure times, and the LC50 and LC90 were calculated. The biocidal effect was assessed by electron microscope. The anti-inflammatory effect was evaluated using two models; the complete Freund’s adjuvant (CFA) induced arthritis and the acetic acid induced colitis model. in albino rats. The Freund’s adjuvant (CFA) induced arthritis was studied using tihirty-six rats (150-200g. body weight) divided into six groups. The first group was kept as negative control. The second group was kept as positive control group. The third group was used as standard group that was given Itrocoxib (90 mg/kg, orally) as standard anti-inflammatory drug. The fourth, fifth and sixth group were treated with Jasminum grandiflorum methanol extract at doses 100, 200, 400 mg/kg body weight, orally respectively. Rat’s paw thickness and rat’s weight were recorded weekly. At the end of experiment samples were collected: blood for serum analysis and paws for thickness assessment. In the colitis model, the animals were divided into six groups (8 rats each). Groups 1 and 2 received vehicle (saline) were kept as negative and positive control, respectively for 7 days. group 3 received prednisolone (reference drug, 2 mg/kg, p.o.), and groups 4, 5 and 6 were given orally TJME at doses of 100, 200 and 400 mg/kg, respectively. On the 8th day, groups 2–6 were administered 2 ml acetic acid solution (4% v/v) in saline intrarectally. Colonic segments, approximately 2 cm proximal to the anus and 8 cm in length, were excised, cleaned and was used for macroscopic and microscopic examination. The lesion scores, ulcer index and percentage of gastric protection of the colon was valuated. The present results revealed that the anthelmintic activity of Jasminum grandifloroum revealed good mortality in parasites with lower concentration as LC50; using the total methanolic extract.The anti-inflammatory activity of J. grandiflorum L. total extract (JTME) showed positive and dose-dependent effects against ulcerative colitis and rheumatoid arthritis. In ulcerative colitis model there was decrease thickness of the mucus layer and intestinal tight junction protein abundances in the colon (P <0.05) by JTME supplementation. Moreover, JTME successfully reduced serum LPS, MPO, MPS levels (as indicators to irritable bowel syndrome) indicating its anti-inflammatory action. In Adjuvant-induced arthritis model, JTME showed apparent anti-arthritis in a dose-dependent manner. , JTME at dose 100 mg/kg group showed improved articular cartilage with partial necrobiotic change and mild inflammatory cells infiltration in the synovial membrane. At dose 200 mg/kg, rats exhibited apparently normal articular surface with mild periarticular edema and inflammatory cell infiltrations. At dose 400 mg/kg rats showed apparently normal joints with subsided inflammation in all examined sections. In conclusion, administration of methanol extract of Jasminum grandiflorum significantly decreased inflammation. It also has parasitical effect against important helminthis and acaricides. Moreover it has anti-microbial activity.The present results proved that Jasminum grandiflorum has anti-inflammatory, anti-colitic, and anthelmintic activities. This is due to presence of phenolic components.