الفهرس 
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Abstract
An experiment was conducted during May 2019 at private farm in el Manyial, Cairo, Egypt. Moringa oleifera plant was treated with four of plant growth promoting rhizobacteria (PGPR) (Bacillus cereus, Bacillus polymyxa, Bacillus megaterium, and Streptomyces chibensis) as biofertilizers to enhance the plant growth, increase yield, improve antifungal and antibacterial activity, total phenolic compounds, photosynthesis pigments, anticancer and antioxidant activity of Moringa oleifera leaves and to replace agrochemicals in the near future. Moringa leaves have varied nutrient quality depending on the microbial inoculants used and the type of inoculation used. The study for effective strains must focus on isolation and screening of individual or combinations inoculants that have multiple traits. Moreover, a better result in microbial inoculant development could be achieved by investigating the different mode of action in disease suppression and plant growth promotion, as well as relationships between the bacteria and various environmental factors. The obtained results showed that treatment by microorganisms in combination is the best choice in improving of Moringa growth and its quality after 65 days of seeds planting in clay and sand soil. The combination of (Bacillus megaterium+ Bacillus polymyxa) showed highest effect in increasing of plant growth parameters and phytochemical character compared with the control. Treatments by bio fertilizing microorganisms (GP4) (B. megaterium, B. polymyxa) in combination exhibited high values of antimicrobial activity against seven food pathogens Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Pseudomonas aeruginosa, Aspergillus niger, Rhizopus stolonifer and Botrytis cinera, as compared with individual treatments and control (untreated plants).
Biofertilization treatment (B. megaterium, B. polymyxa) added to sand soil recorded increasing over control in (GP10) by 160.8% and decreasing of chemical fertilization by 5.41% for polyphenol content, also, the antioxidant content shown increasing over the control (GP10) by 81.1% and decreasing of chemical fertilization (GP9) by 5.6% as well as vitamin C and A were increased with mineral and biofertilizer (GP4) treatments after 65 days. Biofertilization in (GP4) recorded increasing over control group (GP10) by 48.89%, 27.08% and 58% for vitamin A, C and chlorophyll respectively.
While Biofertilization treatment (GP4) added to clay soil recorded increasing over control in (GP10) by 167.84% and decreasing of chemical fertilization (GP9) by 25.07% for polyphenol content, the antioxidant content showed increasing over the control (GP10) by 42.12% and decreasing of chemical fertilization (GP9) by 2.4%. The results clearly indicated that vitamin C and A were increased with mineral and biofertilizer (GP4) treatments after 65 days. Their highest values were obtained using mineral fertilizer treatments followed by biofertilizers as in GP4 treatment. Morever biofertilization in (GP4) recorded increasing over control group (GP10) by 14.8%, 89.91% and 56.78% for vitamin C, A and chlorophyll respectively. Ethyl acetate extract was found to be most potent extract than ethanol 70% and aqueous solvent against all tested food pathogens. Additionally, the ethyl acetate extract of Moringa oleifera leaves inoculated with combined biofertilizers (GP4) showed great cytotoxicity for colon, breast and lung cell line and safe for normal cells. Which strongly suggesting that it could potentially be an ideal anticancer therapeutic candidate specific to various types of cancer cells.
Furthermore, the ethyl acetate Moringa treated with GP4 extract showed best source as antimicrobial agent, also can be used as natural food preservatives for tomato and bell pepper.