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العنوان
Biological control of tomato damping- off disease caused by rhizoctonia solani kuhn =
المؤلف
Bresam, Mohammed Abdulhasan.
هيئة الاعداد
باحث / محمد عبد الحسن بريسم
مشرف / مصطفي عبد العظيم أبو السعود عامر
مشرف / سعد محمود محمد شمة
مشرف / اسلام ابراهيم احمد ابو السعود
الموضوع
Tomato - disease.
تاريخ النشر
2019.
عدد الصفحات
ix,124,3p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم النبات
تاريخ الإجازة
30/11/2019
مكان الإجازة
جامعة الاسكندريه - كلية الزراعة ساباباشا - النبات الزراعي
الفهرس
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Abstract

6.1. Isolation, purification and identification
1. Tomato Damping-off disease caused by Rhizoctonia solani is considered as one of the most destructive diseases in Egypt. six isolates of R. solani. were collected from 6 geographical regions at -Behaira, Alexandria, Monufia, Beni Suef, Faiyum and Kafar Elsheikh. The obtained isolates were, then identified as R. solani.
6.2. Molecular characterization using ITS
2. Used technique ITS for identification Molecular identification of R. solani cultures investigate and confirm from all R. solani isolates.
Appearance results identification different all R. solani isolates.
6.3. Pathogenicity tests
3. The pathogenicity studies showed that the tested tomato cultivars (Freda, Alia, Prencesa, Alex, Jana, Alesa), varied in disease incidence. Alesa tomato cultivar was more resistant than other cultivars. Whereas, the more virulent R. solani isolates were Beni Suef.
6.4. Biological control
6.4.1. Inhibition of biological control agents against R. solani in vitro
4. showed the effect of biological control agents T. viride and B. subtilus on all tested isolates of R. solani, T. viride and B. subtilus were effective to inhibit the growth rate of all tested isolates of R. solani and effect varied from 43.11, 57.05%, 60.46, 50.76, 48.53 and 45.23% with Beni Suef, Faiyum, Alexandria, Monufia, Beheira and Kafr Elsheikh, respectively.
5. The efficacy of T. viride was more inhibition than B. subtilus on all tested isolates of R. solani 57.88 and 43.23%, respectively.
6.4.2. Effect of biological control agents on tomato damping-off disease in vivo
6. The biological activity of bio-control agents has been highly reduced tomato damping-off diseases incited by R. solani in greenhouse experiments. The one highly virulent Beni Suef isolate was increasing the disease incidence comparing Alexanderia isolate soil.
7. In general, the results proved that there was no significant difference between both tested tomato cultivars (Alesa and Freda) for their response to biological control agent tested compared with control.