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Abstract
Vaccination against lumpy skin disease is carried out by heterologous sheep pox vaccine (SPPV) giving un satisfiable results and incomplete protection in vaccinated cattle, since that it was a must to produce a homologues vaccine for LSDV. A local isolate of LSDVstrain was propagated on MDBK cell line and CPE was appeared after 72 hours as cell rounding, cell aggregation and cell degeneration with titer of 105.5TCID50/ml, DNA extraction and PCR for the candidate strain was performed using universal and specific primers for virus identification and confirmation.The size of the produced amplicons was 192 pb for the universal primer and 502pb, 1452pb for the specific primers respectively. Metagenomics is done to evaluate a virus harvest for standardization by applying a suite of genomic technologies and bioinformatics tools to directly represent the genetic content of entire communities of organisms in the given harvest. In this study a 3rd generation sequencer (TGS) was applied using a nanopore technology (MinION) based on identification of DNA bases by measuring the changes in electrical conductivity generated as DNA strands pass through a biological pore (nanopore). Its portability, affordability, and speed in data production makes it suitable for real-time applications, the release of the long read sequencer MinION has thus generated much excitement by producing a high microbial diversity in biological samples that was limited by using second generation sequencing as Illumina