الفهرس 
Title : Studies on preservation of rabbit semen
CONTENTSOnly 14 pages are availabe for public view
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Abstract
The present study was designed to investigate some factors affecting preservation of rabbit semen such as 1) type of the diluent (tris citrate glucose, tris citrate trehalose and INRA82 extenders), 2) type of the cryoprotectant [Dimethyl-sulfoxide (DMSO 8%), dimethyl-formamide (DMF 8%), and a combination of DMSO 4% and DMF 4%], and 3) addition of some antioxidants to semen extender: Bovine serum albumin (BSA 5,10 and 15 mg/ml); Zinc sulphate (Zn 150, 200 and 250 æM ); Arachidic acid (A A 5, 10 and 20 æg/ml) and melatonin (0.5, 1,0 and 1.5 mM). Eight New Zealand white rabbit bucks were used in this study. Evaluation of chilled semen extended in INRA82 was significantly (P{u02C2} 0.05) higher than the other two diluents. So, INRA-82 extender is a promising diluent that can be effectively used to maintain viability and fertility of chilled rabbit semen. Evaluation of post-thaw semen parameters showed a significant (P{u02C2} 0.05) improvement after adding a combination of DMSO 4% and DMF 4% to INRA82 extender before freezing. Supplementation of INRA82 extender with 10 mg/ml BSA or 1.00 mM melatonin or 150 æM Zn or 5æg/ml AA showed a significant improvement of post-thaw semen characteristics (sperm progressive motility, alive sperm, sperm membrane and acrosome integrities and spermatozoa with non-fragmented DNA) and fertility of rabbits. In conclusion, dilution of semen of New Zealand white rabbit bucks with INRA82 extender at rate 1:1 with addition of DMSO 4% + DMF 4% as cryoprotectant agent and BSA 10 mg/ml or melatonin 1.00 mM or Zn 150 oM or AA 5 æ/ml as antioxidant was useful to improve the potential and fertility of chilled or cryopreserved spermatozoa