الفهرس 
Title : Phytochemical and biologicalstudy of madina dates (phoenixdactyliferavar. ajwah) and its phyto-nanoformulation
ContentsOnly 14 pages are availabe for public view
 |  | from | 325 |  |  |
| | | from | 325 | | |
Abstract
Ajwa fruits are among the most nutritious and economically valuable fruits. In this study, the effect of different solvents on the extraction of polyphenols was investigated. Aqueous acetone (50%)extract containedthe highest content of polyphenols and flavonoids. Filtration of the extract through a Diaion HP-20column led to a polyphenols-enriched fraction (Fr1) that had the highest antioxidant activity as measured with ORAC and DPPH assays. Metabolic profiling of the different extracts using HPLC-ESI-QTOF-MS-MS led to the tentative identification of 33 secondary metabolites: 3 phenolic acids, 19 flavonoid glycosides including 4 sulfated derivatives, 2 lignans, and 9 fatty acids. Furthermore, the hydrolysis products of Fr1were studied by HPLC-DAD-UV and revealed presence of quercetin and luteolin aglycones. Fr1showed a promising hepatoprotective activity when tested invitro utilizing HepG2 cell lines and in-vivo against thioacetamide-induced liver fibrosis in mice.Ingestion of Fr1in the form of a microemulsion had significantly higher oral bioavailability than when ingested as a suspension in water