الفهرس | Only 14 pages are availabe for public view |
Abstract Azo dyes are complex derivatives of diazene used in food and textile manufacture. As recalcitrant compounds, they account for major environmental and health problems. The bioconversion of Remazol black B (a commonly used water soluble dye) by Pseudomonas aeruginosa was obtained in static conditions. Different strains of Pseudomonas species were isolated from textile wastewater effluents. The aim of this study was to investigate the potential of pseudomonas aeruginosa to decolorize and degrade textile azo dye and to optimize the decolourization conditions. The effect of inoculum size, pH, dye concentration, EDTA, carbon, nitrogen source, static and shaking conditions were investigated on dye decolourization. The bio-decolourization process was optimized using the multi factorial Plackett-Burman experimental design. A 100% decolourization of 200 mgL⁻¹ was achieved in less than 48 hours. Interestingly, the presence of yeast extract, magnesium and iron in the cultural media, highly accelerated the rate of decolourization. Moreover one of our isolates, P aeruginosa KY284155, was capable of keeping high degradation rates at high pH (pH=9), which represent the pH of most textile wastewater effluent and was able to tolerate high concentration of dye up to 500 mgL⁻¹ |