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Abstract To date, there is no standard therapeutic protocol for the treatment of MTrPs and different alternatives have been suggested. This draw the attention of many investigators to assess and compare some of the therapies used for MTrPs deactivation with a view for establishing more effective alternatives for the treatment of MTrP. Therefore, it was the aim of the present study to evaluate and compare two forms of treatment for inactivation of MTrPs: DN and LA injection. Although the comparison between these two methods was the topic of several studies, their results showed great controversy. 100 eligible patients with active MTrPs within the jaw muscles were randomly allocated to one of two study arms with 1:1 allocation ratio by using computerized sequence generation: the ’intervention’ group who underwent DN of the TrPs or the control group who underwent Mepivacaine injection of the TrPs. Sixty patients dropped out throughout the trial. This resulted in a study sample which consisted of 40 patients, 20 patients in each group, of both genders (37 females and 3 males), aged between 14-50 years (average of 30.17 years). Each patient in both groups received 3 treatment sessions at an interval of 2 days in the first week, 2 treatment sessions at an interval of 3 days in the second week and one treatment session at the end of the third week. For both groups, the evaluation was realized in three moments: preoperatively (T1) as well as one week (T2) and 12 weeks (T3) after the end of the treatment sessions. The primary outcomes were the patients{u2019} current and worst pain intensity and PPT. The secondary outcome was the MMO |