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Abstract SUMMARY AND CONCLUSION This investigation was conducted during two successive seasons (2019 /2020 and 2020/2021) on two young mango cultivars namely, “Sediek” and “Ewais” to determine the effect of foliar spraying with different levels of silicone which applied in the form of potassium silicate “25% SiO 2 +10% k 2 O.” (0.0%, 0.1% and 0.2%) and α- tocopherol “vitamin E” (0 ppm, 200 ppm and 400 ppm) to mitigate the harmful effect of low, high temperatures and negative impact of soil salinity on young mango trees. The trees were about 2 years old at the beginning of study, planted at 5*6 m apart, each cultivar was grafting on Sukkary rootstock "tolerant to salinity" and grown on a sandy loam "newly reclaimed lands and the soil salinity was 4.55 dSm -1 " at the experimental farm of the Fac. of Agric. at Demo, Fayoum, Egypt. The experiment comprised the following treatments: 1: Control sprayed with distilled water only. 2: Sprayed with 0.1% potassium silicate (P.S. 1 ). 3: Sprayed with 0.2% potassium silicate (P.S. 2 ). 4: Sprayed with 200 ppm α-tocopherol (α-Toc. 1 ). 5: Sprayed with 400 ppm α-tocopherol (α-Toc. 2 ). 6: Sprayed with 0.1 potassium silicate (P.S. 1 ) + 200ppm α-tocopherol(α-Toc. 1 ). 7: Sprayed with 0.1 potassium silicate (P.S. 1 ) + 400ppm α-tocopherol (α-Toc. 2 ). 8: Sprayed with 0.2 potassium silicate (P.S. 2 ) + 200ppm α-tocopherol (α-Toc. 1 ). 9: Sprayed with 0.2 potassium silicate (P.S. 2 ) + 400ppm α-tocopherol (α-Toc. 2 ). SUMMARY AND CONCLUSION 2 Our results could be summarized as follow: 1-The obtained results revealed that the growth characteristics like tree height, scion and rootstock diameters, number of leaves/shoot, leaf area and shoot length for mango trees of Sediek and Ewais mange cvs. were negatively influenced by abiotic stresses. This might be due to severe cold of winter and/or summer heat as well as soil salinity which decrease physiological and biochemical processes like nutrient metabolism, ion uptake, carbohydrate assimilation and growth promoters, thus reducing plant growth and development. Consequently, the lowest significant values of growth parameters were detected by young mango trees grown under abiotic stresses conditions without spraying with potassium silicate and/or α- tocopherol. 2-Results showed that tree height increased by increasing application rates of potassium silicate and/or α-tocopherol in the two studied seasons. This may be due to foliar application of P.S. and/or α-Toc. stimulated vegetative growth of mango trees due to the positive effects of silicone on vegetative growth of trees especially under adverse conditions by improving nutrient uptake, mechanical properties of the plant parts and stimulating active roots. Also, silicon and α-tocopherol inhibits oxidative degradation by enhancing the activity of antioxidant enzymes that reduce reactive oxygen SUMMARY AND CONCLUSION 3 species caused by stress conditions and toxic metals. Therefore, the highest values of the tree height (160.67and167.30 cm during April and October 2020, respectively were achieved by tree sprayed with 0.2% Potassium silicate plus 400 ppm α-tocopherol. Meanwhile, foliar application by the lower levels of P.S. (0.1%) and α-Toc. (200ppm) alone or combined gave the lowest values on this respect. 3-The obtained data indicate beneficial influence of spraying with α-tocopherol alone than spraying with potassium silicate individually. Also, trees which were sprayed with α-tocopherol alone gave higher values of tree height, leaf area, scion and rootstock diameters comparing with those sprayed with potassium silicate alone especially at higher level of treatments. 4-The data clearly showed that leaf area was significantly enhanced by foliar application of potassium silicate and α-tocopherol alone or in combination. Trees of Ewais cultivar grown under abiotic stresses and sprayed with foliar application of potassium silicate and α-tocopherol alone or combined produced the largest leaf area comparing with the trees of Sediek cultivar grown under the same conditions. 5- Young mango trees sprayed with a higher concentration of P.S. (0.2%) plus 400 ppm α–Toc. together gave the highest significant values of shoot length (38.01cm and 59.32cm during April and October, respectively (average of the two studied seasons). SUMMARY AND CONCLUSION 4 Followed in descending order by those sprayed with 0.1% Potassium Silicate + 400 ppm α–Toc., 0.2% potassium silicate + 200 ppm α- tocopherol and then by 0.1% P.S. + 200 ppm α-Toc., respectively. 6- Number of leaves per shoot of Sediek and Ewais mango cvs. was affected significantly by spraying with potassium silicate and α-tocopherol alone or in combination. Consequently, the lowest number of leaves/shoot was observed for young mango trees grown under environmental stresses conditions. Also, number of leaves per shoot was increased by increasing levels of potassium silicate and α- tocopherol alone or in combination. 7- Abiotic stresses, including salinity, cold and extreme temperature caused a harmful effect on relative water content (RWC) and membrane stability index (MSI) for young mango trees under study. Young mango trees exposed to the adverse environmental conditions had the lowest significant values of RWC and MSI. On the other hand, foliar application with P.S. and α-Toc. alone or together enhanced significantly these parameters. The negative impacts of abiotic stresses on relative water content and the membrane stability index of young mango trees may be due to the accumulation of reactive oxygen species that causes membrane damage, lipid peroxidation, and hindered physiological and biochemical processes such as nutrient metabolism, ion uptake, and loss of cell water content. SUMMARY AND CONCLUSION 5 Furthermore, silicon protects trees against oxidative damage by maintaining water uptake, forming antioxidant defense, increasing photosynthesis activity and root growth. In addition, antioxidants like alpha-tocopherol play an essential role in protecting the cell membrane from the harmful effects of abiotic stresses by increasing membrane stability, stimulating photosynthesis and decreasing reactive oxygen species and lipid peroxidation. 8-Results reveal that Chlorophyll fluorescence (F v /F m ) and SPAD chlorophyll differed significantly by foliar application of potassium silicate and α-tocopherol alone or together. Leaves of trees sprayed with the higher levels of P.S. +α-Toc. together had the highest significant values of F v /F m and SPAD chlorophyll. Moreover, the effect of spraying with α-tocopherol alone on F v /F m and SPAD chlorophyll was more noticeable than spraying with potassium silicate individually particularly at higher concentration of treatments. 9- The obtained results show that total carbohydrate contents increased by increasing appellation rates of potassium silicate or α- tocopherol alone. Furthermore, total carbohydrate content of young mango trees spraying with α-tocopherol alone was higher than that of trees spraying with potassium silicate alone. Young mango trees sprayed with the higher level of potassium silicate (2%) + 400 ppm α-tocopherol had the highest significant values of total carbohydrate (25.78 % in April and 26.27 in October). Meanwhile, total SUMMARY AND CONCLUSION 6 carbohydrate content of trees sprayed with the lower level of this mixture (1% potassium silicate + 200 ppm α-tocopherol were 23.42 % in April and 23.95 % in October "averages of the two studied seasons". 10- Negative relationships was detected among free proline content in leaves of young mango trees grown under adverse environmental conditions and foliar application of potassium silicate and α-tocopherol singular or together. Consequently, leaves of young mango trees exposed to abiotic stresses without any treatments had the highest significant values of free proline content. Spraying with potassium silicate alone was more noticeable than spraying with α- tocopherol alone especially at higher levels of treatments on free proline content. 11- Phenol content differed significantly by foliar application of potassium silicate and -tocopherol separately or together. As demonstrated by the obtained results, the lowest significant values of phenols (1.05 and 1.07 mg g1 DW in April and October, respectively "averages of the two studied seasons") were detected by untreated trees. In addition, phenol content was raised by increasing the application rate of potassium silicate and/or –tocopherol. 12- In general, leaf minerals content like N, P, K, and Ca as well as the K/Na ratio of young mango trees grown under abiotic stress were enhanced significantly by foliar application of potassium SUMMARY AND CONCLUSION 7 silicate and/or α-tocopherol alone or together comparing to control treatment especially at higher levels. On the other hand, leaf Na content showed an opposite trend under the same condition. Therefore, young mango trees grown under abiotic stress without any treatments had the highest significant values of Na percent. In addition, leaf minerals content like N, P, K, and Ca of young mango trees sprayed with α-tocopherol singularly was the most promising than those sprayed with potassium silicate alone. In the end. It can be concluded that heat, cold and salinity stresses were characterized by inducing a dramatic decline of all physiological responses of young mango trees. Meanwhile, foliar application of potassium silicate and/or α-tocopherol alone or in combination significantly improves morphological, physiological characteristics and leaf mineral content. Foliar application of α-tocopherol alone was more noticeable comparing spraying with potassium silicate alone particularly at the higher rates and maintained the growth and trees performance under abiotic stresses. potassium silicate or α-tocopherol plays a vital role in inhibiting oxidative degradation by enhancing the activity of antioxidant enzymes that reduce reactive oxygen species caused by abiotic stresses and ion toxicity. They also improved cell water status SUMMARY AND CONCLUSION 8 through ameliorating phenolic content, membrane stability index and relative water content, reflecting its vital role as anti-oxidant. According to our results, we recommend foliar spraying with either P.S. at 0.2% or α-Toc. at 400pp alone or combined for four times in winter season (on the first of December, 1 st of January, 1 st of February and then on the first of March). And for four times during summer season (on the 1 st May and after one month later till up the first of August) to alleviate adverse effects of salinity, low and high temperatures on young mango trees. |