الفهرس 
الغلافOnly 14 pages are availabe for public view
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Abstract
In last decades, Mushroom is used widely in feeding for its medicinal properties, many studies confirmed its role as antioxidant, anticancer, antidiabetic, antiallergic, immunomodulating, cardiovascular protector, anticholesterolemic, antiviral, antibacterial, antiparasitic, antifungal, detoxification, also protect against tumor development and inflammatory processes. The aim of the present study was to investigate the effect of mushroom (Agaricus bisporus) as a part of diet on hepatotoxic rats. Thirty adult male albino rats (Sprague-Dawley strain), weighing about (200±10g) were divided randomly into two main groups as follow: the first group ((-ve) control= 6 rats) was fed on basal diet. The second group (24 rats) were fed on basal diet and injected with CCl4, using a five necrogenic dose to induce acute liver injury, then divided into 4 groups from group 2 to group 5. group 2 ((+ve ) control) fed on basal diet. group 3 and 4 fed on basal diet supplemented with 20% of dry uncooked mushroom and 20% of dry cooked mushroom, respectively. Finally, group 5 fed on basal diet supplemented with 10% of double (aqueous and ethanolic) extract of mushroom. At the end of the experimental period (4 weeks), rats were scarified and serum was collected for biochemical analysis and histopathological investigation for liver. Results indicated that mushroom extract had more powerful in phenolic and flavonoid compounds. The major polyphenolic components of mushroom were α-tocopherol, Syringic and chrysoeriol. These results were reflected to the antioxidant activity (DPPH) of mushroom extract were recorded 91.90% at the high tested level 1280 µg/ml of sample. Concerning results of serum concentrations of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and total bilirubin were significantly increased (P< 0.05) in the positive control group compared with the negative control.In the same way, malondialdehyide (MDA) level in liver tissue homogenate was significantly increased (P<0.05). While, decreased in reduced glutathione (GSH), superoxide dismutase (SOD) and catalyzes (CAT). It also indicated that supplemented diet with dry uncooked, dry cooked and its extract reversed these changes that caused by CCl4 administration. It could be recommended that mushroom is worthy treating on hepatotoxicity.