الفهرس | Only 14 pages are availabe for public view |
Abstract Calf diarrhoea is multifactorial disease entity that can have serious financial animal welfare implications in both dairy and beef herds.This study aimed to isolate the bacteria causing diarrhea and biochemical identification of the isolated bacteria and Antimicrobial susceptibility testing for the isolated strains and Molecular characterization and virulence and antimicrobial resistance genes of the isolated bacteria using PCR. A total of 215 fecal samples were obtained from diarrheic calves were tested in this study, including 175 buffalo calves and 40 cattle. The incidence varied amongst farms, ranging from 0% to 27.9% pervelance. 37.2 % of calves were 1 to 7 days old, 55.8 % were 7 days to 3 months old, and 6.9 % were older than 3 months. Bacteriological testing, antibiotic susceptibility testing, and PCRbased detection of certain virulence and antimicrobial resistance genes were all performed. E. coli (85.5 %) , C.perfringens (8.8%)and Salmonella (3.7%) were the most common bacterial infections recovered from calves. All Salmonella strains tested positive for the invA gene, while all C.perfringens strains tested positive for the cpa gene. The majority of the identified strains were resistant to clindamycin, erythromycin, and oxytetracycline. The retrieved strains harbored blaTEM,qnrA, and tetA. Approximatly,96.2 %of E. coli isolates were multidrug-resistant (MDR) to various antimicrobial classes, 62.5 % of Salmonella isolates were multidrugresistant (MDR) to different antimicrobial classes, 10.5 % of C.perfringens were extensive drug-resistant (XDR) to seven antimicrobial classes, and 84.2 % of C.perfringens were multidrugresistant (MDR) to various antimicrobial classes. |