الفهرس 
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Abstract
Abstract
Equine herpesvirus-l (EHV-l) is endemic in many areas of the world including Egypt. It is one of the infectious viruses affecting family equidae causing epidemic abortion, neonatal mortality, respiratory diseases and neurological disorders. This study aimed to isolate the EHV-1 with antigenically and molecular identification of circulated isolates in Giza. The study was applied on seventy-two samples from which 30 tissue samples (aborted fetal tissues, fetal fluids and placenta) were collected from aborted mares of native breeds at different ages during 2018 - 2020) and 42 vaginal and nasal swabs were collected from aborted mares and adult horses of different ages with a history of respiratory manifestations. The samples were processed and a trial for virus isolation was done on Vero cell culture. Positive samples showed a characteristic cytopathic effect for EHV-1. Identification of positive EHV-1 isolates was done by fluorescence antibody technique (FAT). The study revealed that 12.5% of the samples were positive for EHV-1. The TaqMan real time PCR confirmed the obtained results. Sequencing and phylogenetic analysis revealed that these isolates were closely related to each other and those previously isolated from Egypt and to other European EHV-1 strains. It is concluded that, the molecular characterization using PCR methods and phylogeny analysis enhanced the detection of the circulating EHV-1 in Egypt. leading to broaden the understanding of the virus epidemiology in the field and implicate strong control and preventive measures.