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العنوان
Molecular Evaluation of Some (Phaseolus vulgaris L ) Genotypes using DNA barcoding /
المؤلف
Ali , Mayada Youssef El-Sayed.
هيئة الاعداد
باحث / ميادة يوسف السيد علي
مشرف / محمد سراج الدين عبد الصبور
مناقش / محمد حسن رفعت
مناقش / تامر احمد العقاد
الموضوع
Molecular Evaluation Genotypes using DNA barcoding
تاريخ النشر
2022
عدد الصفحات
110 p.:
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الزراعية والعلوم البيولوجية (المتنوعة)
تاريخ الإجازة
1/1/2022
مكان الإجازة
جامعة بنها - كلية الزراعة - كيمياء
الفهرس
Only 14 pages are availabe for public view

from 132

from 132

Abstract

ABSTRACT
Among the different biological sources, the common bean, and the wild type (Phaseolus spp.) Have many biotechnological applications. The DNA barcode of the use of the rbcL gene has proven its usefulness in the study of Phaseolus spp. Phylogenetic diversity of genotypes, more than a cryptic introduction, environmental modulation, geographic distribution, and species identification in exclusive bean species. Eleven samples of common bean had been obtained from unique locations in Egypt. The gene of rbcl has proven successful in PCR reactions. The identification of the species published that the samples of the specimen River garow, Samantha , Scala , Nambale , Goru , Cerdon, Bronco , Giza-3, Gomi , Concessa and Valantino belong to Phaseolus vulgaris, and two samples of the specimen Teparry113, Teparry116 belong to Phaseolus acutifolius, respectively. The rbcL-based DNA barcode was once nearly profitable to discover distinctive specimens of Phaseolus spp according to species and genus. Some samples of the rbcL gene were no longer sufficient to perceive the stage of the genus and could not differentiate between relatively comparable species.
Key words: Barcoding; rbcL gene; phylogenetic diversity; Common Bean; Phaseolus spp.