الفهرس | Only 14 pages are availabe for public view |
Abstract ABSTRACT Among the different biological sources, the common bean, and the wild type (Phaseolus spp.) Have many biotechnological applications. The DNA barcode of the use of the rbcL gene has proven its usefulness in the study of Phaseolus spp. Phylogenetic diversity of genotypes, more than a cryptic introduction, environmental modulation, geographic distribution, and species identification in exclusive bean species. Eleven samples of common bean had been obtained from unique locations in Egypt. The gene of rbcl has proven successful in PCR reactions. The identification of the species published that the samples of the specimen River garow, Samantha , Scala , Nambale , Goru , Cerdon, Bronco , Giza-3, Gomi , Concessa and Valantino belong to Phaseolus vulgaris, and two samples of the specimen Teparry113, Teparry116 belong to Phaseolus acutifolius, respectively. The rbcL-based DNA barcode was once nearly profitable to discover distinctive specimens of Phaseolus spp according to species and genus. Some samples of the rbcL gene were no longer sufficient to perceive the stage of the genus and could not differentiate between relatively comparable species. Key words: Barcoding; rbcL gene; phylogenetic diversity; Common Bean; Phaseolus spp. |