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Abstract Contents of fiber and benzoic acid derivatives may be the key principles of the variations between maize and DK as feed resources. Maiz-based diet exhibited higher GP in the first few hours of incubation than the DK diets, while at 24 h all diets produced similar GP values for all the experimental diets. The experimental diets interacted with the ruminal inocula initial pH for most end fermentative products as well as nutrient degradability. All DK diets were able to modify SCFAs fermentative profile to produce higher C3 and lower C2 than maize-based diet, and this phenomenon was pH-dependent. Substitution of maize by DK up to 50% was the most recommended for ruminants because it can maintain rumen pH unchangeable without a rapid decrease and thus may prevent further inadequate ruminal buffering capacity caused by high fermentative grain diets. This study also highlighted the importance of considering the original pH of the ruminal fluid before the addition of the buffering nutritive solution in the in vitro assays. Still, DK mode of action to reduce CH4 emission needs further evaluation under different feeding in vivo conditions in long term experiment. |