الفهرس 
ENGLISH ABSTRACTOnly 14 pages are availabe for public view
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Abstract
The bone defects produced by an infection, a trauma hamper or totally inhibit the clinical recovery. Presently, only few available intervention measures are successfully applied to overcome those challenges. The tissue engineering technique has been applied for managing the wound healing process to ensure that the tissue can be regenerated.
The present research was intended for investigating the osteo-matrix actions on the critical-sized defect healing of the rabbit’s calvarial bone.
This present study’s null hypothesis was that there weren’t any significant differences between both the presence or absence of bio-composite material in accelerating of the bone healing process in the calvarial critical-sized bone defect in rabbit.
Our study included eighteen male Egyptian Rabbits with an average of one year of age and weighing 3.5-4 Kg. Animals will be obtained from the animal house of Medical Research Institute, Alexandria University. The rabbits were kept under the same environmental conditions in the experimental animal house. The histological procedures were done in the laboratory of the Oral Biology Department at the Faculty of Dentistry, Alexandria University.
All of the operative techniques were performed under general sedation. The rabbits will be anesthetized by intramuscular injection of (0.15-0.20mg)/Kg ketamine plus (1-2mg)/Kg lidocaine. The surgical area in the calvarial of all the rabbits was shaved before any procedure and the skin is rinsed and scrubbed with 2% povidone iodine to avoid contamination.
For each rabbit: two defects were created the right (control) and left (study) using 8 mm diameter sterile surgical bur (Trephine bur) with profuse saline irrigation to protect bone from heat generation. The control defect was left empty while the study defect received biocomposite material osteomatrix crumb SOFIKAR company (bone collagen, bone sulfated glycosaminoglycans (sGAG) and hydroxyapatite in natural form). The surgical area was sutured by 000 non absorbable braided sutures. All rabbits received the same course of antibiotics amoxicillin 1gm/Kg BW every 8 hrs. for 5 days.
Specimens were fixed in neutral buffered formalin at a 10% concentration, cleaned, and then decalcified. The samples were cleaned, dehydrated in progressively stronger alcohol, clarified in xylene, and then infused with and embedded in paraffin wax. Using a rotary microtome, 5 m thick serial sections will be cut from the paraffin blocks and stained with hematoxylin and eosin. The stained slices were examined under an optical microscope to determine whether any new calcified bone had formed. To compare the proportion of the newly produced bone’s surface area in various groups, histo-morphometric evaluation of the newly formed bone was carried out using Image J 1.46r software.