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Abstract Bond stability relies on the mechanical characteristics of dentin collagen fibers. Grape seed extract (GSE), which is rich in proanthocyanidin (PA), has the ability to enhance dentin stiffness by stimulating collagen crosslinking. Objectives: The study was conducted to measure the in vitro effect of 6.5% PA on the dentin shear bond strength of resin-modified glass ionomer cement (RMGIC) and composite resin and to evaluate its effect on the dentin bonding interface using scanning electron microscopy (SEM). Materials and Methods: Twenty-four primary teeth were collected, randomly and equally allocated into 2 groups according to the treatment of dentin: group I (n=12): 6.5% PA was applied to the dentin before applying the restorative materials and group II (n=12): 6.5% PA was not applied to dentin. Both groups were subdivided into 2 subgroups (n=6) according to the restorative materials used: RMGIC and Composite resin. Shear bond strength was determined using a Universal Testing Machine. The debonded area was then assessed under a stereomicroscope to evaluate the mode of failure. Additional 16 teeth were collected for assessment of dentin bonding interface by Scanning Electron Microscopy. Teeth were equally and randomly assigned to groups (n=8) and subgroups (n=4) as mentioned before. Results: As regards the shear bond strength, no statistically significant difference was detected with the application of PA compared to the control group using both restorative materials, while a statistically significant difference was found between the restorative materials. Shear bond strength of composite resin was significantly higher than RMGIC in both test and control groups (P>0.001). Regarding the mode of failure assessed in the fractured specimens, mixed and adhesive failure patterns were predominantly observed in all groups, with no significant difference observed between them (PMC=0.72). Conclusion: PA did not significantly increase the dentin shear bond strength of composite resin and RMGIC. |