الفهرس 
Mesenchymal Stem CellsOnly 14 pages are availabe for public view
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Abstract
Background: Mesenchymal stem cells (MSCs) and hepatocytes are considered valuable candidates for cell-based therapy. Using free zoonotic media as purified platelets derived growth factors (L-GF) and human platelet lysate (hPL) instead of using fetal bovine serum (FBS) to support the growth and proliferation of these cells could be used as a promising therapeutic tool in hepatic regeneration. Aim of the work: To evaluate the usage of purified platelet derived growth factors and platelet lysate in both stem cell and hepatocyte cultures and compare it to the usage of FBS. Methods: Culture of stem cells and hepatocytes in growth media supplemented with L-GF or hPL and compared them to their culture in growth media supplemented with FBS. Cells were subjected to population doubling (PD) and generation time (GT) calculations. Results: The best result for MSC was that obtained by using 10% hPL or 10% FBS with the highest cell count, highest viability and shortest incubation time needed to reach confluency compared to supplementation with 10%, 20% or 30% L-GF. As for hepatocyte culture, the use of 10% FBS for supplementation of media used for hepatic cell proliferation showed better performance regarding cell count, viability and incubation time to reach confluency compared to the use of either hPL or L-GF. Conclusion: 10% hPL had the best results in stem cell culture which suggests that hPL might be a better alternative for the development of xenofree stem cell culture that can be used for many clinical applications. On the other hand, 10% FBS showed the best results in hepatocyte culture.