الفهرس 
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Abstract
In this current study, we evaluated the influence of BMMSC-CM on the HepG2 cell line and attempted to describe the possible underlying molecular signaling
pathways involved.
We used male albino rats for the isolation of BMMSCs.
Then we identified those cells using culture morphology
and specific surface markers of BMMSCs (CD44, CD90,
and CD105) by flowcytometry. We then prepared the
BMMSCs-CM to be used in this study. We treated HepG2
cells with multiple concentrations (20%, 40%, 60%, 80%,
and 100%) of BMMSCs-CM over different durations of
time (24 hours, 48hrs, and 72hrs). The MTT assay was
used to determine the viability of HepG2 cells after
BMMSCs-CM treatment, and it showed significantly
decreased viability as the concentration was increased and
as the time of treatment was prolonged. Flowcytometric
analysis of apoptosis revealed an increased apoptotic rate
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with cell cycle halt in the G0/G1 phase along with an
inhibitory entry in the S phase. RT-PCR evaluation of P53
and Bcl-2 mRNA levels showed the upregulation of P53
and the downregulation of Bcl-2. Furthermore, protein
level analysis of P53 and Bcl-2 by flowcytometry and
western blotting revealed increased P53 and decreased
Bcl-2 protein levels. In addition, ELISA results revealed
increased ROS production and decreased TLR4
expression.