الفهرس 
Vitamin DOnly 14 pages are availabe for public view
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Abstract
Abstract:
Background and Aim: Immune thrombocytopenia (ITP) is an acquired immune- mediated disease of adults and children that is characterized by excessive platelet destruction and decreased platelet production. Several studies have demonstrated the role of Vitamin D receptor (VDR) polymorphism in the development of autoimmune diseases.
This study aims to find the relationship between vitamin D receptor gene (BsmI) polymorphism and response to treatment with steroid therapy in primary immune thrombocytopenic patients.
Subjects and methods: We selected fifty patients with primary immune thrombocytopenia (ITP), divided according to their response to steroid therapy into 2 groups, respondent and resistant patients. VDR polymorphisms (BsmI) was genotyped and used to evaluate the association of VDR variants with ITP. VDR polymorphism BsmI was detected by PCR followed by restriction fragment length polymorphism analysis.
Results: This study enrolled fifty patients with primary immune thrombocytopenia (ITP), divided according to their response to steroid therapy into 2 groups, respondent and resistant patients. The study shows that the mean Vit D in Steroid Responder group was (20.12) and in Steroid Resistant group was (15.28) with high statistical significant difference in between (P=0.0013). As regarding to vitamin D receptor gene polymorphism (BsmI) analysis there was a high statistical significant difference in between the two groups (P=0. 0064). BsmI (BB) allele was the dominant allele in patients who showed response to steroid therapy (44%), while (Bb) was the dominant allele in the resistant patients (60%).
Conclusion: Vitamin D receptor (BsmI) polymorphism was found to be associated with higher incidence of ITP and can be used as a risk marker for ITP susceptibility in adults. There was a highly significant between of VDR BsmI genotype frequency in ITP patients and the post treatment platelets count in the respondent and resistant patients. Patients carrying BsmL (Bb) allele showed resistance to steroid therapy while patients carrying BsmL(BB) allele showed good response to steroid therapy