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Abstract Background: Mechanical neck pain is a widespread significant health problem with a huge burden on the individual and economy. Finding a non-invasive effective treatment that is non-exhaustive for the therapist and non-painful for the patient is highly needed. Study design: Double-blinded randomized clinical trial Objective: To investigate and compare the effects of myofascial trigger point release (TPR) and instrument-assisted soft tissue mobilization (IASTM) using M2t blade in terms of neck pain level, pressure pain threshold (PPT), neck lateral flexion and rotation range of motion and neck function on upper trapezius myofascial trigger points (MTrPs) in mechanical neck pain. Methods: Forty patients between 18 and 55 years were randomly assigned to either group A or B. group A received one session of Trigger point release (TPR) and passive stretching while group B received one session of IASTM using M2t blade and passive stretching. Visual analogue scale, Pressure algometer, measuring tape were used to evaluate patients{u2019} pre-treatment, post-treatment, and at follow up. Arabic version of the neck disability index (NDI) was used pre-treatment and at follow up. Results: There was a significant improvement in all outcomes immediately post-treatment and at follow up in both groups (p < 0.001). There was a significant increase in PPT and decrease in VAS and NDI levels in favor of group B at immediate post-treatment and at follow up (p < 0.01) without significant difference at lateral flexion and neck rotation between both groups (p > 0.05) |