الفهرس 
Molecular genetics studies of resistance of some plants to nematodes
Abstract EnglishOnly 14 pages are availabe for public view
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Abstract
Root knot nematodes, especially Meloidogyne incognita, is one of the most economically important pests in Egypt and worldwide. Thus, it is very important to understand the molecular mechanisms underlying plant-nematode interaction to find the key regulatory genetic elements that control and enhance plant resistance against nematodes. As M. incognita life cycle is mainly divided into three stages, i) invasion ii) feeding cells formation and iii) reproduction, it is important to study and elucidate the different responses of the plant against nematodes at each life cycle stage. Phytohormones are molecular signals that are produced in the plants at low concentrations but play important roles in nearly all plant development processes along with regulating plant defenses against different pests and pathogens. This study focuses on examining the different responses of phytohormones, such as salicylic acid (SA), 12-oxophytodienoic acid (OPDA), jasmonic acid isoleucine conjugate (JA-Ile), and abscisic acid (ABA) using LC/MS, against M. incognita during the different life cycle stages in tomato (Solanum esculentum), cv. Money Maker as a susceptible plant.The levels of the phytohormones at the three-life cycle stages were measured using liquid chroma-tography (LC). In addition, the differential expression of the phytohormone-related marker genes such as PR1, Pin2, and LapA were quantified using qRT-PCR. The results showed increased levels of SA concen-trations and upregulation of PR-1 gene at the feeding cells formation stage compared to that in the invasion and reproduction stages. While, OPDA concentrations were high along with the up-regulation of corres-ponding gene LapA at the invasion stage in comparison to those at the feeding cells formation and repro-duction stages. JA-Ile concentrations were high at the production stage and pin2 gene was up-regulated during the feeding cells formation stage