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Abstract ABSTRACT This study was carried out at a private farm in AL-Radisiya city, Markaz Edfu, Aswan Governorate, Egypt on the banks of River Nile and Experimental laboratory of Horticulture Department, Faculty of Agriculture, Benha University, EL-Qaloubia Governorate, Egypt during the two successive seasons of 2018/2019 and 2019/2020 on Chia (Salvia hispanica L.) plant. A study was initiated through various bio stimulant and chemical fertilizer aiming to evaluate effectiveness of Arbuscular Mycorrhiza Fungi (AMF) or seaweed extract (Spirulina platensis ) and/or humic acid as well as chemical fertilizer (N.P.K) and the combination of the treatments mentioned above on vegetative growth, seed yield, chemical constituent’s, fixed oil productivity and fixed oil composition of chia (Salvia hispanica L.) plant. Plants were sprayed with biostimulants gave the significantly highest mean values for all of studied characteristics mentioned above, particularly T6: the combined of mycorrhiza, humic acid, Seaweed and recommended dose of N P K followed by T5: recommended dose of N P K (350:200:100 kg/fed) in the two seasons. Meanwhile, T4: Seaweed (1cm/L) ranked the third values in parameters mentioned afore in this concern. Whereas, the richest fixed oil percentage were scored by T6, followed discerningly T5 in the 1st and 2nd seasons. chromatography analysis of chia fixed revealed the identification of four components, which identified, i.e. palmitic acid, oleic acid, linoleic acid and α-linolenic acid. The main component was α-linolenic acid (43.34 to 53.28 %). It can be concluded that T6 was the best for improving growth, seeds yield, fixed oil productivity, chemi |