الفهرس 
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Abstract
Bacopa monnieri commonly known as “Brahmi” is an important medicinal herb of the family Scrophulariaceae. Bacopa is a medicinal plant of high commercial potential with legendary reputation as a memory vitalize. Many different studies have showed that Bacopa is useful in treating Alzheimer’s disease, anxiety, and age-related cognitive decline. In the present work, optimization and effort to improve the existing protocol for the in vitro culture conditions of Bacopa monnieri for mass multiplication, callus culture and hairy root using Agrobacterium rhizogenesis study has been initiated. MS solid medium supplemented with BA at 1.0mg\l without IAA gave the best shoot number (271.66 shoots/explant), shoot length (7.00 cm), growth rate (4.33) and multiplication rate (27.33). largest shoot number (428.33shoots/explant), growth rate (4.66), multiplication rate (43.00) and the minimum shoot length (2.33 cm/ explant) were resulted by MS solid medium + (0.5 Kin+0.0 IAA) mg/l. BA at concentration 0.25mg/l with 1mg/l IBA gave the best shoot number (482.66 shoots/explant), growth rate (5.00) and multiplication rate (48.33) with MS solid medium but, MS liquid medium supplemented with 0.25 mg\l IBA alone gave the longest shoot length (5.50 cm/ explant). BA at concentration 0.50mg\l with 0.50mg\l NAA gave the best shoot number (139.00 shoots/explant) and multiplication rate (14.33) with MS liquid medium. Maximum shoot number per explant was recorded on full strength medium, which was significantly higher than other medium strengths.
The maximum leaf number (830.00 leaf/explant) and node number (415 node/ explants) were observed on the medium solidified with 3g/l agar and 1g/l gelrite combination. But the same medium recorded the minimum shoot colour.
Light intensity at 2000 lux produced the highest values of shoot number (149.75 shoot/explant), leaf number (578.75) and multiplication rate (15.00).
20g\L glucose gave the largest shoot number (148.75 shoot\explant), growth rate (4.25) and multiplication rate (15.00) with MS solid media.. The best shoot number (17.00 shoot\explant), leaf number (76.00) and node number (38.00) resulted from two nodes horizontally position. The best shoot length (4.12cm) occurred due to one node horizontally position. The best of root number (23.00root\explant) resulted from three nodes horizontally method. The highest percentage of callus formation (100) was obtained by culturing of leaf on MS medium contained all concentration of 2,4-D and KIN except 0.25, 0.5 or 1.0mg/l 2,4-D and control were recorded lower response. The highest number of root (57.00) and number of shoot (40.50) was formed on 1.00 mg/l IBA. On the other hand, the highest shoot length (7.12cm\explant) and leaf number (237.50) were formed on 0.75mg/l IBA than other treatments. The best number of roots were recorded (23.40) on a mixture of 1 perlite+3peatmos, number of plant (19.60) on 2 perlite+3peatmos, length of root (6.40cm) on 1perlite+2peatmoss and length of plant (17.40) on 1 sand+2 peatmoss mixture after three months. Wounded or non-wounded explant with co cultivation using the strain A4 showed maximum transformation frequency, (80.00%) and hairy root length (5.96cm) after 60 min. from infection period of wounded explant. Using wounded method with Agrobacterium rhizogenes LBA9402 for 60 min. gave the maximum hairy root % (81.67) and length of hairy root (4.76cm) for 60 min. of infection period.