الفهرس 
Title : Implementation of Digital Pathology in Diagnosis of Some Neoplastic Lesions in Pets
AbstractOnly 14 pages are availabe for public view
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Abstract
Fifty-three (53) tumor samples were collected from pets{u2019} clinics in Cairo, Egypt for a year (Jan 2019-Jan 2020) to study their histological characters and to be used as a digital pathology (DP) module for teleconsulting and compare between light and virtual microscopy to validated virtual microscope use in primary diagnosis of pets affections. Tissue specimens were routinely prepared into slides and stained with hematoxylin and eosin (H&E) for histopathological examination. The 1st part of samples was 12 feline mammary glands tumor (FMT) the histological parameters of each sample were carefully identified, and the diagnosis of some samples was confirmed using special stains as Masson{u2019}s trichrome (MTC) and immunohistochemical (IHC) staining as anticytokeratin CK8/18 and vimentin markers. Overall, the studied cases were heterogenic histologically and the most frequent histological pattern was tubulopapillary carcinoma. There were 3 rare mammary tumor cases; glycogen rich, mucinous and lipid rich carcinoma. All malignant samples were CK8/18 positive and one displayed growth of pleomorphic myoepithelial cells that was positive for vimentin marker. The 2nd part of samples was 12 canine mammary gland tumor (CMT), the frequently detected type was mixed tumors and the histologically predominant pattern was solid carcinoma. CMT samples with myoepithelial growth were confirmed by MTC, vimentin and P63 markers. Grading of CMT was done according to Elston and Ellis grading system. The last part of samples were 29 skin tumor masses. Five samples were of adnexal origin, 3 samples were basal cell carcinoma (BCC), 3 were papilloma, one was squamous cell carcinoma (SCC), 7 were round cell tumors of lymphoma and transmissible venereal tumor, 8 samples were soft tissue tumors and the last 2 remaining samples were hepatoid gland adenoma and carcinoma. MTC stain was used to stain fibrous tissue and vimentin IHC staining confirmed fibrosarcoma