الفهرس 
Title : Some studies on brucellosis in sheep and goats in Gulf region
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Abstract
This study was performed to determine the sero-prevalence of sheep and goats (small ruminants) brucellosis in some areas of Arabian Gulf, using by media-culture, serological methods (Rose Bengal Plate test (RBPT), indirect enzyme-linked immunosorbent assay (i-ELISA), complement fixation test (CFT) plus blood polymerase chain reaction (PCR) and PCR for tissue samples from positive reactors. from 140 (73 sheep and 67 goats) tissue samples were collected and examined using media culture, it was found that, out of the 140-tissue sample collected, 67/140 (47.8%) were confirmed positive, and biovar 1 is dominant in area 1 and 3, while biovar 2 is dominant in area 2, The serological examination using RBPT revealed that the Infection rate was total (855/20067 (4.26%)) animals were sero-positive, by using i-ELISA, and CFT reveledthat out of the 855 RBPT-positive reactors, positively confirmed in 717/20067 (3.57%), 595/20067 (2.97%) and respectively, were confirmed as positive.By PCR blood for confirmation cleared out of 855 positives by RBPT, 264/20067 (1.32%) animals, from confirmed positive reactors of animal 140 (73 sheep and 67 goats) tissue samples were collected and examined using PCR, collected from the positive reactor (RBPT and CFT), it was found that, out of the 140-tissue sample collected, by using PCR found that out of the 140-tissue sample collected, 128/140 (91.4%) were confirmed positive. PCR also can differentiate between vaccinated rev-1 strain and field strain B. melitensis (field infection strain)