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Abstract Background: The major genetic causes in male infertility are chromosomal abnormalities and Y chromosomal microdeletions (YCMs). YCMs occur in approximately 15% of azoospermic patients and 10% of severe oligospermic patients. These microdeletions lead to spermatogenic failure. Aim of study: This study aims to investigate the incidence of AZF microdeletions in Egyptian infertile males with severe oligoospermia & non obstructive azoospermia (NOA) using multiplex PCR. Patient and methods: One hundred-fifty infertile males were included. Semen analysis, hormonal assay, karyotyping, testicular sperm extraction and testicular biopsy were performed. Y chromosome microdeletions were detected by using multiplex polymerase chain reaction (PCR). Result: Among 150 infertile males; Considering Y chromosome; 3/36 (8.3%) in severe oligoospermic group while 21/114(18.4%) of non-obstructive azoospermia (NOA) had Y chromosome microdeletions in specific AZF subregions. Conclusion: The frequency of Y chromosome microdeletions in the studied patients was similar to many ethnic reports. Detection of AZF microdeletions is necessary for proper genetic diagnosis in infertile males. AZFc can help informed decisions regarding positive TESE outcome |