الفهرس 
Title : Assessment of sperm DNA fragmentation and oxidative stress in couples with recurrent implantation failure
AbstractOnly 14 pages are availabe for public view
 |  | from | 116 |  |  |
| | | from | 116 | | |
Abstract
Recurrent implantation failure (RIF) is defined as the failure to achieve pregnancy following 2{u2013}6 in vitro fertilization (IVF) cycles or intracytoplasmic sperm injection (ICSI). Recently, some evidences correlate the integrity of sperm DNA to RIF have been recorded.To investigate this suggestion, the sperm DNA fragmentation in partners of 110 women with recurrent implantation failure (RIF) following intracytoplasmic sperm injection (ICSI), and 50 recent fathers (control) were examined. Sperm DNA fragmentation was examined by the sperm chromatin dispersion (SCD) test, Aniline blue test (AB), Acridine orange (AO) test and DNA diffusion assay. The study also examined different aspect causing DNA fragmentation such as oxidative stress that measured by GSH and MDA, ROS that measured bydichlorofluorescin diacetate.There were no obvious differences in sperm DNA fragmentation index measured among different used tests. However, AB was found to provide clear and stable clinical cut-off levels, and considered as the most easy and inexpensive assay. Therefore, AB can be recommended for a clinical sperm DNA damage evaluation as a routine test. Moreover, impaired chromatin packaging, apoptosis and ROS result in significant DNA damage in human spermatozoa of RIF group compared to control and positive pregnancy groups.These results support the hypothesis that sperm DNA fragmentation is an important cause of RIF, and that sperm DNA integrity testing has value as a diagnostic tool in investigation and treatment of infertility. Keywords: Recurrent implantation failure; DNA fragmentation; Oxidative stress; Apoptosis and ROS generation