الفهرس | Only 14 pages are availabe for public view |
Abstract It is known that silver nanoparticles (Ag NPs) and silver nitrate AgNO3 have harmful effects on the surrounding organisms, which may cause damage to these organisms. Therefore, the aim of this study is to detect damage caused by Ag NPs and silver nitrate to land slugs (Lehmannia nyctelia). In this study, the slugs were exposed to various concentrations of Ag NPs and AgNO3 for 15 days. The biochemical, antioxidant, lipid peroxidation (LPO), DNA fragmentation, and histopathological endpoints were assessed after 15 days of exposure to different concentrations of Ag NPs and silver nitrate (0.04, 0.08, 0.4, and 0.8 g/L). The results showed a significant decrease in total protein, total carbohydrate, superoxide dismutase, and GST and a significant increase in total lipid, LPO, and DNA fragmentation after exposure to Ag NPs and AgNO3 for 15 days compared with the control group Histological examinations were performed in each digestive gland, kidney and gonads. The digestive gland appeared after exposed to different concentrations (0.04, 0.08, 0.4, 0.8 g/L) a clear change in the shape of the cells which the cells became more flat and some many cells were damage. The size of the cavity inside the tubules increased and filled with some remains of damaged cells and the cells became empty from inside. The histological study of the kidney revealed a regeneration in some cells, widening of the spaces between the tubes (gaps) and an increased in connective tissue size than the normal size. |