الفهرس Only 14 pages are availabe for public view
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Abstract
Blow flies are the first insect group to colonize humans and animal corpses.They deposit their eggs on corpses and aid in the decomposition process. Identifying an insect specimen is the first and the most important criteria in forensic entomological analysis.The stages and species of insects observed on a dead body, such as Calliphoridae, give evidence for estimating the minimum postmortem interval (PMI).This study aimed to identify and describe a newly collected Calliphora vicina from Damietta, Egypt, using the light microscope and 2barcoding area3 of the mitochondrial DNA cytochrome oxidase I gene (COI) as a general marker to distinguish forensically significant adults. More morphological detail along the male and female body was drawning by using Camera Lucida.These details can be morphologically valuable for easy identification of this fly in Egypt.There was no significant difference in this study of the morphological characteristics of Calliphora vicina species between specimens from Egypt and those from Africa. DNA was extracted from ten adult Calliphora vicina, and a ” " ~ " ”760 bp region of the COI gene was amplified, visualized on a 1% agarose gel, and sequenced. The tree with the most elevated log probability is (-2252.70), and a proportion of sites had at least one unambiguous base present in at least one sequence, resulting in a descendent clade in the tree. This investigation included 55 DNA arrangements, and codon positions included were 1st+2nd+3rd+Noncoding.This research highlights the requirement for careful testing to determine all possible genetic assortments if DNA barcoding continues to be utilized for molecular identification