الفهرس | Only 14 pages are availabe for public view |
Abstract Ziziphus spina-christi leaves (ZSC), Rhamnaceae, and Euphorbia umbellate latex (EU), Euphorbiaceae, considered being of the common potent natural. So, the aim of study was to carry out a bio-monitored investigation of extracts using in vitro assay. Well-developed nanostructure incorporated with these extracts into a unique form with greater biocompatibility was investigated. The study demonstrated a combination of polysaccharide-based nanoparticles with extracts into a unique form which prepared by using two biocompatible polymers; alginate (ALG) and chitosan (CS). To investigate potential interactions between ALG/CS-NPs and the extract (ZSC/EU), physicochemical characterization was performed. The release profiles of phenolic compounds from ZSC/EU loaded ALG/CS-NPs were evaluated using quantification method. ZSC/EU loaded ALG/CS-NPs was developed as a potent immunomodulatory natural extract with promising antimicrobial activity against multidrug resistant bacteria, antiviral activity against Coxsackie B3 virus, antioxidant activity against free radicals and oxidative stress, anti-inflammatory activity by inhibitory effect on lysosomal enzymatic activities and anti-proliferation activity against DNA oxidative damage (hepatocellular carcinoma cell line The results indicated that the novel agent has potent antioxidant activities and potent inhibitory effect on lysosomal enzymatic activities |