الفهرس 
Title: The effect of temperature on age estimation of semen stain on porous and non porous surfaces using messenger ribonucleic acid (mRNA
List of ContentOnly 14 pages are availabe for public view
 |  | from | 147 |  |  |
| | | from | 147 | | |
Abstract
Background: mRNA can be used to identify the type of a body fluid and the mechanism of its degradation can give some indication of the time it was deposited. Semen is one of the body fluids which is considered as reliable marker in sexual assault cases as they contain nucleic acids that aid in relating an assailant and/or a victim to a crime. Seminal stain can be affected by the surrounding environment, such as environmental temperature, humidity, the media on which the stain is deposited, either by preserving the sample or by degrading it. Degradation is the main obstacle that faces the investigators, as degradation may negatively affect the nucleic acid that carries all information that will assist in solving the case.Objectives: The aim of this study was to evaluate the effect of temperature on age estimation of human semen stain using mRNA at different time intervals and on porous and non-porous surfaces. Methods: Ten semen samples obtained from Andrology outpatient clinic, Faculty of Medicine, Cairo University. Semen was directly deposited into sterile containers it was of normal quantity and quality.Samples were applied on 2 different media (Glass & Cotton) and exposed to 3 different temperatures (4{u00B0}C, Room Temperature & 40{u00B0}C) and examined at 3 time intervals (day0,day45 &day 90).Semen specific mRNA markers (PRM1 & PRM2) where assessed along with a reference gene (Beta actin) and quantitatively detected by real time RT-PCR