الفهرس 
Title: A Study on characterization and Identification of a Vitamin Producing Bacteria Isolated from Gut and their Impact on Host Metabolism In Vivo
List of contentOnly 14 pages are availabe for public view
 |  | from | 137 |  |  |
| | | from | 137 | | |
Abstract
This study was conducted to isolate, identify and characterize Lactic acid bacteria as vitamin producers, especially B2, riboflavin and B9, folic acid vitamins.A total of 150 fecal samples were collected from newborn - 60 years old voluteers. The collectd fecal samples were a source for the isolation of probiotic vitamin producers. Fourty nine isolates showed probiotic characteristics were chosen for screening their genome by PCR for the presence of riboflavin and folic acid genes. Consequently, three isolates were selected for their productivity of the B2 and B9 as confirmed by HPLC. The three isolates were identified to the species level by sequencing their 16S rRNA gene which showed 100% identity to strains of Pediococcus acidilactici. Thus, they were named as Pediococcus acidilactici WNYM01, Pediococcus acidilactici WNYM02, Pediococcus acidilactici WNYM03 and submitted to the Genebank database with accession numbers.The three isolates were tested in vivo on rat colitis model to test their anti-inflammatory effect and the result compared to control group.The histological examination of the rat intestine showed the anti-inflammatory role of our probiotic strains compared to the control group. Such finding, may sugest the potential novel of these three probiotic candidates for pharmaceutical