الفهرس 
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Abstract
In the current study, 11 samples from tuberculin positive cattle were identified as M. bovis and 8 samples from human sputum samples from clinically confirmed cases with tuberculosis infection were subjected to bacteriological isolation and biochemical identification. PCR was used to genetic identify the isolates, the IS6110 gene specific to mycobacterium tuberculosis compelx. The RD8 region specific to M. tuberculosis only. An isolate from the bovine sample (M. bovis) was subjected to phagosome like environment to study the gene expression profiling of M. bovis under the stress of the immune – cells (using hydrogen peroxide) which play a very important and crucial role in competing tuberculosis infection Using two color Microarray. Statistical analysis using the GX V13 software revealed that out of 15000 probes covering the whole genome of mycobacteria ( three probes per gene) there were 105 genes up regulated and 21 genes down regulated and other genes were differentially unchanged in the phagosome like environment.