الفهرس 
Advanced studies on Toxoplasmosis in small ruminants with reproductive disorders
AbstractOnly 14 pages are availabe for public view
 |  | from | 81 |  |  |
| | | from | 81 | | |
Abstract
Toxoplasmosis is an infectious disease caused by protozoan T. gondii which causes reproductive failure in small ruminants. Newly developed methods as Genotyping is essential to explain the virulence, epidemiology, and effective treatment and control in human and animals. This study was conducted to assess the seroprevalence of T. gondii in sheep and goats in Egypt especially those with a history of reproductive disorders including abortion and molecular identification of T. gondii followed by studying the genetic variety of T. gondii isolates rotating in Egypt. Blood samples were collected from 138 and 212 live ewes and she-goats from five Governorates of Egypt. Also, the blood and its corresponding tissue samples were collected from 180 ewes and 206 she-goats from (El-Moneib, El-Warak and El-Basatin abattoirs) at Cairo, Giza governorates of Egypt. All samples were serologically examined using ELISA and the tissue samples of the seropositive animals were digested and microscopically examined then bio-assayed in mice. All the T. gondii isolates were subjected to molecular identification using PCR and genotyped using nested PCR (n-PCR) combined with restriction enzyme analysis (RFLP) of the SAG2 gene. The total seropositivity of live ewes and she-goats was 26.8% and 21.2%, respectively, while in slaughtered ewesandshe-goats was 16.6% and 33%, respectively. 66.6% and 53.3% of slaughtered ewes, 26.4%, and 17.6% of slaughtered she-goats were positive using microscopic examination and mice viability test, respectively. Toxoplasma gondii tissue cysts were detected with associated characteristic histopathological changes of different organs. Twenty-eight T. gondii isolates were confirmed using PCR. Among 24 milk samples from seropositive live ewes and she-goats, only 12.5% and 6.25%, were positive using PCR respectively. Genotyping confirmed 26 isolates (92.8%) as type II, 2 (7.1%) as type III. Here, Type II and III were the genotypes mostly circling among small ruminants in Egypt and this is the most significant for public health in Egypt