الفهرس 
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Abstract
A total of 410 milk samples were collected from different ruminant species (110 cows,
87 buffaloes, 108 does and 105 ewes), belonging to different farms. The samples were
investigated for L. monocytogenes and the results revealed the prominence of the
bacteria in the examined milk of apparently healthy cows, subclinical mastitic cows
and apparently healthy goats in percentages of 1.72 %, 2.77 % and 1.38 %.
respectively. The recovered isolates were identified biochemically and phenotypically
by positive hemolytic reaction and motility, followed by molecular confirmation using
specific primer sequences of 16s rRNA. Antibiogram of the L. monocytogenes isolates
demonstrated resistance to tetracyclines, lincomycin, streptomycin, cefotaxime,
trimethoprime sulfamethoxazole, florphenicols, and clindamycin. Adherence assay
using polystyrene microtiter plates concluded that L. monocytogenes was able to form
a strong biofilm.