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Abstract Bioethanol has been classified as the most widely utilized biofuel in the world because it helps greatly to decrease crude oil consumption and pollution. Thus, this study was trended to bioconversion of lignocellulosic biomass (lowcost substrate)to bioethanol with the aid of cellulolytic fungi and Saccharomyces cerevisiae. Firstly, pretreatment of lignocellulosic biomass was done to facilitate its digestibility. Secondly, fungi produce multicellulolytic enzymes to convert cellulose into soluble reducing sugar. Finally, soluble sugar was fermented into bioethanol by Saccharomyces cerevisiae. Thirty-one fungal species were isolated from different places in Al-Gharbia and Aswan governorates |