الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Infectious bursal disease (IBD) and infectious pancreatic necrosis (IPN) are two economically important viruses belong to Birnaviridae that threats poultry and fish industry in Egypt. In this study, a total of 8 chicken bursal samples from 8 broiler farms in Fayoum 2021, Egypt suspected IBDV infection were collected and examined by RT-PCR for IBDV and IPNV using specific forward and reverse primers targeting VP2 gene. Four chicken
bursae are positive for IBDV and yielded a specific band of 620 bp amplicon. However, all chicken bursa are negative for IPNV and Five wild ducks were collected from Rashid
coasts and examined by RT-PCR for IBDV and IPNV using specific forward and reverse
primers targeting VP2 gene. One duck bursa are positive for IBDV and yielded a specific
band of 620 bp amplicon. Meanwhile, all duck bursa are negative for IPNV. A total of 94
marine fish samples of five different fish species were tested for IBD-VP2 and IPN-VP2
gene. Spleen, hepatopancreas and kidneys of each species were pooled in separate container
(12 pools) and RNA were extracted. All fish pools are examined by RT-PCR for IBDV and
IPNV. All examined fish are negative for IBD-VP2 gene and IPN-VP2 gene. PCR products
of chicken IBD-VP2 of two Fayoum 2021 strains were selected for sequencing (G4 and
G7) and phylogny. G4 and G7 sequences are completely identical to each other with
identity percentage of 100% at the levels of nucleotide and amino acid sequences indicating
the same origin. Percentages of nucleotide identity between Fayoum IBDV strain and other
Egyptian strains (11 strains) ranged between 97.2 % to 100%, meanwhile Fayoum IBDV
strain are closely identical to Alexandria and South Sinai strains with identity % of 100%
and 99.61 % respectively indicating that it may originated from that sites.