الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Thyroid cancer is most common endocrine malignancy and papillary carcinoma (PTC) is the most common type of thyroid malignancy, accounting for ” ” " ~ " ” ”80 % of all thyroid cancers. Thyroid cancer incidence has tripled over the past three decades owing to detection of subclinical cases. Several changes in healthcare practice have led to the increased detection of thyroid cancer through easier access to the health care system, increased use of high-resolution ultrasound examination, more frequent biopsy and surgery, and more thorough analysis of tissue by pathologists.
TSH receptor (TSHR) is the primary molecule regulating both differentiated function and growth of thyroid follicular cells. Single nucleotide polymorphisms are the most common variation among individuals determining their susceptibility for different diseases.
Our aim was to study the association of thyroid stimulating hormone receptor gene polymorphism (rs179247 and rs12101255) with differentiated thyroid cancer and their relationship with clinicopathological characteristics of these tumors.
The study was conducted on fifty patients with documented PTC. Patients with thyroid nodules were selected from Alexandria University hospital outpatient clinics after excluding hyperthyroidism, autoimmune thyroid diseases, and non-suspicious nodules. Eligible patients were subjected to total thyroidectomy in the head and neck and endocrine surgery unit. DNA, extracted from formalin fixed paraffin embedded normal and malignant tissues, was analyzed by real time polymerase chain reaction using TaqMan® Genotyping Assay. No difference was found between normal and malignant tissues regarding TSH receptor polymorphism. No relations were found in both polymorphisms with clinical and biochemical characteristics of the patients. Rs179247 genotype AA (vs. AG and GG: P=0.044*) was associated with extrathyroidal extension. In conclusion rs179247, and not rs12101255, may predict extrathyroidal extension in papillary thyroid cancer.