الفهرس 
TABLE OF CONTENTS
Islets of LangerhansOnly 14 pages are availabe for public view
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Abstract
The present work aimed to study the histological changes induced by diabetes in pancreatic β-cells, and PI3K-Akt pathway expression in pancreas using a pre- and post-treatment schedule with NaB in juvenile diabetic albino rats (aged 5–6 weeks) A total number of 60 juvenile albino male rats (80–90 g) was used in this study and divided into six equal groups: group I: untreated control rats received saline. group II: NaB control rats; received NaB dissolved in saline and administrated by IP route at a dose of 500 mg/kg/day for 21 days. group III: (STZ 3 days) diabetic control rats; induced by single IP injection of STZ (60 mg/kg) then left for 3 days before sacrification. group IV: (STZ 21 days) diabetic control rats; induced by single IP injection of STZ (60 mg/kg) then left for 21 days before sacrification. group V: (NaB + STZ 3 days); rats were pre-treated with NaB (500 mg/kg/day) via IP injection for 21 days prior to diabetes induction.group VI: (STZ 21 days +NaB); rats were induced diabetic by single IP injection of STZ (60 mg/kg), left for 21 days then post-treated with NaB (500 mg/kg/day) via IP injection for 21 days. Blood samples were collected from the retro orbital veins then the samples were centrifuged and the plasma was separated and stored at -20 C for measurement of plasma glucose and plasma insulin levels. To asses glucose homeostasis, IPGT was performed and AUC was calculated for statistical analysis Small specimens from pancreatic tail were taken from all groups and prepared for light (using H&E and Masson’s trichrome stained sections) and electron microscopic examination. The morphological changes were confirmed with IHC using caspase-3, Ki-67 and anti-insulin antibodies. Acetylated H3 antibody was used to ensure HDAC inhibition potential of NaB.