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العنوان
Taxonomic and molecular studies on family
Hydraenidae (Coleoptera: Staphylinoidea) and
putatively associated Wolbachia lineages in
Egypt /
المؤلف
Bayoumi, Ahmed Reda Ismaieel Mohamed.
هيئة الاعداد
باحث / احمد رضا إسماعيل محمد بيومي
مشرف / حسن حمدنا الله فضل
مشرف / عمرو احمد محمد
مشرف / جوهره مجدي محمد أبوالحسن رمضان
تاريخ النشر
2021.
عدد الصفحات
234 P. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم الحشرات
تاريخ الإجازة
1/1/2021
مكان الإجازة
جامعة عين شمس - كلية العلوم - قسم علم الحشرات
الفهرس
Only 14 pages are availabe for public view

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Abstract

This work deals with the taxonomic, molecular, and
phylogenetic studies on the Egyptian fauna of family Hydraenidae
and covers the following points:
1- Taxonomic studies:
A comprehensive taxonomic study was carried out
on family Hydraenidae in Egypt. This study was based on
the species collected through visits to 15 governorates
during the period from 2018-2020, as well as the specimens
preserved in the main Egyptian Reference Collections and
those recorded in literature. According to this study:
▪ Our fauna comprises 17 species within three genera;
Ochthebius Leach, Limnebius Leach, and Hydraena
Kugelann which are belonging to two subfamilies
Hydraeninae and Ochthebiinae.
▪ Two newly recorded species (Ochthebius micans BalfourBrowne and Hydraena arabica Balfour-Browne) are
identified.
▪ Six species were doubted to be included in the Egyptian
fauna (Hydraena quadricollis Wollaston, Ochthebius
difficilis Mulsant, O. rugulosus Wollaston, O. minimus
Fabricius, Limnebius frontinalis Balfour-Browne, and L.
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nitidus Marsham) according to the world catalogue of
insects by Hansen (1998) and the catalogue of Palearctic
coleoptera by Jäch (2004).
▪ Keys and diagnoses are given to all taxa. Type localities,
synonyms, specimens examined, local & geographical
distributions, distributional maps and photos for each
species are provided.
2- Molecular and Phylogenetic Studies:
A. Hydraenidae
The PCR product of CO1 gene was approximately
680 bp. The PCR products of Hydraena arabica,
Ochthebius lividipennis, O. merdionalis, and O.
thermalis have been sequenced from both forward and
reverse directions. The evolutionary relationship among
the four hydraenid species was demonstrated by a
phylogenetic tree based on the partial nucleotide
sequences of CO1 gene, and this phylogenetic tree
revealed that these species are clustered into two major
groups.
B. Wolbachia
Our Wolbachia phylogeny clarifies the clustering
between non taxonomically related beetles, which
indicates the lack of coleopteran host specificity to
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Wolbachia and proves the low degree of codivergence
between Wolbachia and beetles’ ancestors. Besides,
there is an obvious correlation between Wolbachia
supergroups B and F within the beetles’ host, this tree
also indicates that supergroup F is the common ancestor
of the other Wolbachia supergroups